Using of Comet Assay to detect DNA Damage in Infertility Men

Authors

  • Ghassan Mohammed Hassan College of Agriculture- Wasit University, Iraq

Keywords:

comet assay, damage DNA

Abstract

Male infertility is a major health concern and is often accompanied by abnormal semen parameters. The detection and understanding of these abnormalities are critical in the improvement of fertility treatments and outcomes. This study aims to compare semen quality between men with infertility and healthy controls, focusing on sperm count, motility, morphology, and other important semen parameters.

A cohort of infertile men and healthy control donors were evaluated for semen parameters. Semen volume, sperm count, motility, vitality, and morphology measurements and the analysis were performed by using standardized laboratory procedures. The statistical differences were determined by applying independent t-test, Mann-Whitney U test, and descriptive analysis.

The semen volume in men with a diagnosis of infertility was significantly reduced compared to the control group (1.17 ± 0.50 ml versus 3.39 ± 0.56 ml, p < 0.0001). The sperm count in infertile men was also significantly lower (8.15 ± 4.02 x10⁶/ml versus 79.33 ± 9.20 x10⁶/ml, p < 0.0001). Moreover, sperm motility and vitality were significantly reduced, with motility being 24.75 ± 10.10% and active sperm at 1.73 ± 0.86%, compared to controls (76.26 ± 9.05% and 21.33 ± 9.02%, p < 0.0001). Abnormal sperm morphology was increased in infertile men (91.03 ± 3.25% vs. 10.46 ± 3.97%, p < 0.0001), while normal morphology was significantly lower. Semen morphology parameters (Q2 and Q3) showed wide variability within the infertility group, though differences between groups were not statistically significant.

Men with a diagnosis of infertility showed significant semen-quality anomalies, presenting decreased sperm count, motility, and vitality, and an increased incidence of atypical sperm morphology. Our findings underline the necessity for developing better diagnostic tools in terms of DNA integrity assessment and tailored therapeutic interventions for male infertility treatment. Further studies are required on large populations to investigate the clinical consequences of these observations

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Published

2024-11-14