Effect of In Ovo Injection by Crude and Nano-Steroidal Extract of The Bacopa Monnieri L. in Embryonic development and some productive traits of broiler chicks

الملخص

      This study was carried out to understand the effect of hatching egg injection (In Ovo injection) with the Crude and Nano-Steroidal Extract laboratory prepared of the wild Bacopa Monnieri L. in some measurements and embryonic tests about 3 days of embryonic growth, as well as, the Some productive characteristics for the hatched chickens Until marketing, Steroidal compounds were extracted from the plant with HPLC testing to identify some of these compounds and their concentration.  A 705 hatching eggs from the broiler chicken mothers of the Ross 308 at 0 days was used in this study, while the eggs were divided into five treatments of a141 eggs /treatment by (47 egg/replicate) and the experimental treatments were as follows: The First treatment (T1) was negative control treatment without injection, while the second treatment (T2) positive control injects 0.2 ml of N.S (Normal Saline 0.9%)/egg. The third treatment (T3) injects 0.2 ml of N.S containing of 100 μg of Nano Chitosan Cs TPP/egg, while the fourth treatment (T4) injects 0.2 ml of N.S containing 100 μg of steroidal extract /egg. Finally, the fifth treatment (T5) injects 0.2 ml of N.S containing 100 μg of the Nano-Steroidal Extract on Nano Chitosan (Cs TPP)/egg. The results of the experiment showed a significant increase (p in the length of embryo in (T4 and T5) treatments compared to T2 but the two treatments (T1 and T3) didn’t record any significant differences with the other treatments. diameter transparent area recorder Significant increase (P<0.01) for (T1 and T5) compared (T2 and T3) as for T4 didn’t recorder significant difference with the all treatment. (T3, T4 and T5) treatment recorder a high Significant increase (P<0.01) in the diameter of vascular area compared (T1), while treatment (T2) didn’t record any significance with the rest of the treatment. no observed significant difference in measuring (somites) between all treatments. (after hatching) showed a significant increase (P<0.01) body weight average of the chicken for the T5 treatment and in the total weight live gain of the chicken for the T4 and T5 treatments and the significant improvement (P<0.01) in the total feed conversion ratio for the T3, T4 and T5 treatments compared control treatment.