Inducing systemic resistance in rape plants using some bio-elicitors against blight disease caused by Alternaria barassicae

الملخص

This study aimed to determine the effect of the effectiveness of some biological stimulation agents, such as the bacteria Pseudomonas fluorescens and Bacillus sp. the Trichoderma viride fungus and its filters, treated and untreated with heat, reduce the infection of rape plants with blight disease caused by the fungus Alternaria barassicae, which is transmitted through seeds. The results of the study showed that the stimulating biological factors and their filtrate, treated and untreated with heat, have a high inhibitory capacity towards the growth of the pathogenic fungus in the P.D.A. culture medium, as the zone of inhibition for the Trichoderma viride fungus and its filtrate, untreated and heat-treated, reached 100%, 81.14, and 67.33%, respectively. While the antagonistic ability of Bacillus spp. The rates for the untreated and heat-treated filtrate were 100%, 93.58%, and 86.84%, respectively, and it reached 100% when using the P. fluorescence bacterial filtrate and the untreated and heat-treated filtrate which reached 90.22%.

As for the pot experiment, the factors led to the stimulation of systemic resistance and the emergence of significant differences between the percentage of infection and its severity between the treatments and the treatment of the pathogenic fungus. The best treatment was the addition of the bacteria P. fluorescence, as the percentage of infection (%) and its severity reached 0.00%, followed by the treatment of the bacterial filtrate. It reached 1.33 and 2.27%, respectively, while in plants infected with the pathogenic fungus it reached 36.67 and 53.40%, respectively. Treatment with chemical and biological stimulation compounds also improved the rates of some production indicators and some vegetative growth indicators.