Evaluation of the Inhibitory effect of Ganoderma Extracts against the Pathogenic Fusarium Wilt on Potato Plant

الملخص

This study conducted to evaluate the antifungal activity of Ganoderma lucidum extracts against Fusarium oxysporum isolates, a significant pathogen affecting potato crops. Eighteen F. oxysporum isolates were identified and isolated, with three specific isolates' nucleotide sequences registered in the NCBI Gene Bank with accession numbers Mw292595, Mw292596 and Mw292597. The antifungal assays revealed that the Ganoderma lucidum culture filtrate, particularly at 50% concentration, significantly inhibited the growth of F. oxysporum isolates F.o1, F.o2, and F.o3 by 53.00%, 44.50%, and 39.30%, respectively. Phenolic and alkaloid extracts derived from the mushroom substrate also exhibited substantial antifungal activity, with the most potent effects observed at a 5% concentration, achieving inhibition rates up to 49.67%. Field trials corroborated the in vitro findings, with the phenol extract treatment improving plant height, tuber mass, and count, indicating the extracts' dual role in pathogen suppression and plant growth promotion. These results conclude that Ganoderma lucidum extracts could serve as an effective and sustainable alternative to chemical fungicides in the management of Fusarium wilt in potatoes.