The effect of some microorganisms on exDNA traps of lettuce roots Lactuca sativa

Abstract

The study was done in the winter of 2023-2024 in the wooden canopy of the College of Science/University of Al-Qadisiyah with the aim of investigating the effect of some microorganisms on the Root Extracellular Traps of the root environment of lettuce plants. The study included the isolation of two types of fungi, one of which is pathogenic, which is Aspergillus niger, which was isolated from soil samples by dilution series, and the other is a beneficial fungus, which is Trichoderma spp. It was also isolated from the soil by dilution series. Five seeds were planted per pot at a depth of 1.5 cm in 2 kg plastic pots filled with a mixture of soil and peat moss (1:1). After the lettuce sprouted, each seedling was transferred to a separate plastic pot. Extracellular DNA was isolated and extracted from the lettuce roots with and without fungi in the laboratories of Al-Qadisiyah University/College of Science. The results showed that deoxyribonucleic acid secreted from the root border cells is indeed present, as the DNA is secreted in the root periphery. The laboratory results confirmed the presence of mitochondrial DNA, plastid DNA, and plant DNA in lettuce Lactuca sativa. Real-time PCR technology was used to detect the amount of extracellular DNA secreted for each treatment. Trichoderma spp. Results similar to the condition of the plant without addition, while the percentage of extracellular DNA decreased in the presence of the fungus Aspergillus niger.