Study the Therapeutic Effect of Grape-Flavored Whey Probiotic Beverage on Hypercholesterolemia induced in male laboratory rats

Abstract

The present study was conducted in the college of Veterinary Medicine/University of Basrah to evaluate the role of Grape-Flavored Whey Probiotic Beverage that prepared by mixing probiotic isolate (L. acidophilus) and whey in reducing the harmful effect of high cholesterol diet on laboratory rats. Therefore  twenty four adult male rats weight 220-280 g were used and divided into four groups(6 for each). Control group (T1) received normal ration and drench with normal saline. The second, third and fourth groups(T2,T3 and T4 respectively) received high cholesterol diet for 30 day and then drenched by normal saline , whey, therapeutic syrup (5 ml) respectively for 30 days. Blood samples were collect and separate serum immediately for lipid profile (Tch,TG,LDL, VLDL and HDL) analysis. In addition , liver enzymes activity (ALT and AST) and renal function indicators were measured. The role of insulin, leptin and adiponectin also evaluated. The results revealed to the role of therapeutic syrup in reducing the values of lipid parameters (Tch, TG, LDL and VLDL) and improvement of LDL levels. The liver enzymes activities showed significant improvement due to administration of therapeutic syrup of probiotics while urea and creatinine appeared non- significant for all groups. Grape-Flavored Whey Probiotic Beverage administration revealed significant decrease in insulin, leptin and adiponestin levels compared with other hypercholesterolemic and control groups. Therefore,  Grape-Flavored Whey Probiotic Beverage had ameliorative effect against the prognosis of high cholesterol diet on body physiology.