Study of behavior pathway and composition of Hyoscyamus albus L. plant across grith stages

Abstract

          An experiment was conducted to determine the metabolic pathway of tropane alkaloids (Hyoscyamine and Scopolamine) in white henbane plant (Hyoscyamus albus L.) during vegetative growth and flowering stages. Various amino acids were adapted to investigate their impact on the metabolic pathway, the concentration of alkaloids. The study found that the concentration of Hyoscyamine was the highest in the roots compared to Scopolamine, while; its concentration was the lowest in the fluorescence and floral tips. Conversely, Scopolamine concentration was continued to increase in the shoot (floral tips) and the roots starting from the vegetative growth stage (S1) to the floral growth stage (S2), while the opposite trend was observed in plant leaves. The pathway of hyoscyamine alkaloid began in the roots and it accumulates in the leaves and later in the floral tips. Although alkaloid concentration increased positively in both roots and leaves, it exhibited an inverse pattern in the floral tips during the flowering stage and seed maturity (S2). Amino acids affected alkaloid concentration whether used individually or in combination with the influence of plant growth stages noticed, Ornithine proved the most effective amino acid among others. Understanding the metabolic pathways of alkaloids enables the selection of optimal strategies for plant management and to determine the best time to harvest plant parts containing the highest alkaloid concentrations during their ideal growth stage.